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It is typically administered as a daily subcutaneous injection, ideally on an empty stomach

Thus, these data suggested that 2-DG interfere with normal proIGF-1Ea glycosylation process leading to an accumulation of an under-glycosylated form which is still able to fold and secreted by the cells

The presence of the alternative Eb- and Ec-domains hampers the proIGF-1Eb and proIGF-1Ec sensitivity to N-glycosylation inhibitors and determines the nuclear accumulation of prohormones The proIGF-1Eb and proIGF-1Ec ran at the expected molecular weight of ~16.5 kDa and ~12.5 kDa in SDS-PAGE gels respectively, suggesting that these prohormones did not undergo posttranslational modification (Supplementary Fig