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Intracellular crystalline particulate material was found in an ultrastructural study in five of 20 investigated FISS, and in one of the five cases was identified as aluminium-based material (Madewell et al., 2001)

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3.2 Geographical effects Climatic conditions and the pollen ripening period influenced the quality of pollen and the vitality of pollen during the years of cryopreservation