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Cytokine analysis, performed 8 h after administering the modified mRNA to mice with induced SP-B expression, demonstrated a 2-fold and 6-fold reduction in IFN- and IL-12 secretion, respectively, compared to unmodified mRNA

Philp D, Badamchian M, Scheremeta B, et al

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Dithiobis(succinimidyl acetate) 56,57 SS(NHS-CM) 2 N , N -Dicyclohexylcarbodiimide (1.42 g, 6.87 mmol) was dissolved in anhydrous tetrahydrofuran (30 mL) under argon and cooled to 0 C with stirring

The restraints were derived from (i) NOE restraints from intermolecular and intramolecular cross-peaks in NOESY spectra (described in NMR spectroscopy section), (ii) TALOS derived p53 TAD2 and FOXO4-DRI backbone dihedral angles, (iii) hydrogen bond restraints (regions p53 residues 4752, FOXO4-DRI residues 918