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Pyruvate Pyruvate, along with its corresponding metabolic enzymes, can restore energy balance by entering the TCA cycle and therefore regulate genes associated with skeletal muscle differentiation and degradation, ultimately impacting cancer cachexia (Fig

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Electron microscopy sample preparation and data acquisition A 3 l volume of 5 mg ml 1 purified SLC33A1 MAP was mixed with Pmab1 Fv-clasp at a 1:1.5 molar ratio and 20mM GSSG, incubated for 30 min on ice and then applied to glow-discharged Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil) or Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 grids before being plunged into liquid-nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV (FEI Thermo Fisher)

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