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Messenger J, Clark S, Massick S, et al

It induced the expected NLRP3 inflammasome-mediated activation of CASP1, and the cells secreted IL18, but not IL1B and did not induce significant release of LDH

Obesity-induced Endoplasmic Reticulum Stress Causes Chronic Inflammation in Adipose Tissue

While there is no way to completely avoid these confounds (192), these processes are greatly curtailed at 80C but not at 20C, meaning handling speed and storage temperature are crucial