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At predetermined time points (2, 6, 12, and 24 h post-administration), the mice were euthanized, and the residual gel on the skin surface at the application site was removed by washing with PBS

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Unlike PDT, limited by shallow light penetration, or SDT, which suffers from relatively low sonosensitizer-to-ROS conversion efficiency despite improved depth access, the RT/RDT strategy leverages X-rays exceptional tissue penetration and strong ionizing capacity

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