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In the classical ferroptosis regulatory pathway, GSH is depleted by erastin or other system Xc- inhibitors, which reduces cysteine synthesis and leads to GSH synthesis disorder [2, 72, 73]

For the FOXO4-DRI treatment group, 50 M FOXO4-DRI (MCE, HY-P4157) was added to the glucose-free DMEM (Gibco, Catalog #11966-025) medium, followed by 3 h of oxygen-glucose deprivation (OGD) treatment

These bonds are strong, but they aren't invincible

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