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A peer-reviewed study in the Journal of Endocrinology confirming that AOD9604 produces lipolysis and increases fat oxidation in obese mice via the beta-adrenergic pathway, with effects comparable to full-length HGH but without the insulin-disrupting side effects provided early mechanistic validation that informed the human trials

By preventing nicotinamide methylation, the compound increases intracellular NAD+ availability, which in turn activates sirtuins and other NAD+-dependent enzymes critical for metabolic homeostasis

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