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Contrary to expectation they discovered that the resistance was determined by spontaneous and heritable mutations independent of exposure to the phage which resembled a pattern of natural selection

This non-active form of B12 needs to be converted in the body to an active form, versus the readily active version Methylcobalamin

Smart Image Examples Embodiment 1 [0087] Example 1: Preparation of Intermediate a [0088] Weigh Boc-Ala-OH (9.5 g, 50 mmol), HBTU (19.0 g, 55 mmol) and HOBT (8.1 g, 50 mmol) and dissolve in 250 mL of acetonitrile, add DIPEA (13.5 mL, 100 mmol), and activate at room temperature for 5 mins

doi: 10.1074/jbc.M114.553438 54 DonohoeDRGargeNZhangXSunWO'ConnellTMBungerMKet al