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Molecular mechanisms underlying Ca2+/Calmodulin-dependent protein kinase kinase signal transduction

Following the injection, in vivo imaging was conducted using an IVIS Spectrum (PerkinElmer) at various time points: 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, and 48 h post-injection, utilizing an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm