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Despite these limitations, our results suggest that supercooled RBCs stored at -4 C in E-Sol5 represent a good compromise between a sufficiently slower metabolism that preserves both energy and antioxidant reservoirs, without inducing excess hemolysis observed at -8 C

Complementary DNA was synthesized from total RNA with the High-Capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA)

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These medications can help the body make more insulin in response to food, reduce the amount of glucose the liver makes, and slow down digestion

After killing, pancreata were perfused with 500 l RNA Later (Ambion) and snap frozen in liquid nitrogen