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One microgram of RNA was reverse-transcribed into cDNA using HiScript III RT SuperMix for qPCR (+gDNA wiper

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Immunity (2016) 45:90316

Such studies move closer to the goal of precision therapy for asthma by targeting intracellular nodes, such as ERK1/2, rather than broad anti-inflammatory agents, and hold promise for improved management of severe and therapy-resistant forms of this prevalent and debilitating disease