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To address the complications associated with traditional surgery, numerous technological advancements have been introduced over the years, shifting from open surgery to minimally invasive techniques, such as laparoscopic surgery, which reduces blood loss and lowers morbidity (Fig

Artesunate can be used to treat malaria and is derived from artemisinin, which is one of the main components of the herbal medicine Artemisia annua (Mancuso et al., 2021)

Cloning and plasmid construction Deletions of genes were performed by allelic exchange using primers listed in S3 Table and the pKSV7 plasmid [32]