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Additionally, echocardiographic results demonstrated that EVs-siTfR treatment could not mitigate the cardiac function impairment in post-MI mice, as evidenced by comparable function indicators including LVEF, LVFS, LVESV and LVEDV between mice injected with PBS and EVs-siTfR (Fig

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Membranes were blocked with Tris-buffered saline (TBS) and 0.1% Tween-20 (TBS/T) containing 5% bovine serum albumin (Sangon Biotech Co., Ltd., Shanghai, China) at 37C for 2 h, and incubated overnight at 4C with primary antibodies (Abcam, Cambridge, MA, USA

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