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Then, 2 days later, the cells were centrifuged at 300 g for 5 min, snap-frozen in liquid nitrogen and stored at 80 C (3 days after transfection)

Patients with galactosemia present a galactose-1-phosphate uridyl transferase deficiency manifested by accumulation of galactose and its metabolites in the body, resulting in ovarian cell swelling and dysfunction (21)