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*Correspondence: Hongliang Zhang, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

Ideal experiments would simultaneously quantify, with high spatiotemporal resolution, key parameters such as: (1) lactate and glucose dynamics in astrocytes and neurons using genetically encoded biosensors

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Additionally, research by Yu et al (138) suggests that TSA alleviates cerebral ischemia-reperfusion injury by downregulating TGM2, inhibiting PANX1 expression and suppressing microglial activation and neuroinflammation (TNF-, IL-1 and IL-6), highlighting the multi-target regulation of TSA in the same disease

However, nickel affinity purifications are frequently used as a first step in an overall purification protocol that might also include ion-exchange, hydrophobic-interaction, or size-exclusion steps