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Description

Aging gastropathy-novel mechanisms: hypoxia, up-regulation of multifunctional phosphatase PTEN, and proapoptotic factors

[DOI] [PubMed] [Google Scholar] 51.Allegra M., Tesoriere L., Livrea M.A

GST-tagged, 6xHis-tagged or GST-6xHis purified proteins were used as baits and GST was used as control by immobilization on a 96-well MaxiSorp plate (Nunc) overnight at 4 C while shaking (10 g of protein in 100 L PBS)