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Transfection and treatments To screen for CBS aggregates in cells, 8 10 3 cells per well were plated into CellCarrier 96-well microplates (PerkinElmer, Germany, #6055300)

Pentadecapeptide BPC 157 and the duodenal lesion

Carboxyamidomethylation (Unimod4) and methionine oxidation (Unimod35) were designated as fixed modifications, while N-terminal protein acetylation was treated as a variable modification