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The phosphorylation of ubiquitin and Parkin triggers a series of structural remodelling for Parkin, converting it from a dormant enzyme with self-inhibited conformation to an active and promiscuous E3 ligase which mediates ubiquitination of OMM proteins non-selectively 29,31,32,33

Anrather, J

Protein disulphide isomerase protects against protein aggregation and is S-nitrosylated in amyotrophic lateral sclerosis

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The advantage of CBP tags is that the binding and elution conditions are mild, helping to improve the ability of the fusion protein to retain its native form after purification