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In the search for broader anti-tumor strategies, targeting cellular energy pathways has emerged as a central focus of the scientific community and is currently being pursued through extensive preclinical and clinical investigations

Pain intensity ratings using the visual analogue scale (VAS), Hospital Anxiety and Depression (HAD) Scale, and short form-36 health survey (SF-36) questionnaire were filled out by both FM subjects and HC

An evaluation of the role of TDLN in PD-1/PD-L1 checkpoint blockade therapy in two mouse tumor models reveals that immune checkpoint treatment induces CD8+ T cell accumulation in the tumor-draining but not in non-draining LNs (210)abrogated checkpoint therapy, suggesting that TDLNs function as sites of T cell invigoration required for PD-1/PD-L1 checkpoint blockade therapy (211, 212)

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Data from all experiments were processed and analyzed using FlowJo software v.10