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Neuroprotective effect of pseudoginsenoside-F11 on permanent cerebral ischemia in rats by regulating calpain activity and NR2A submit-mediated AKT-CREB signaling pathways

The suspension was diluted by adding 400 L of buffer containing 150 mM NaCl, 1 mM MgCl 2 , and 50 mM Tris-HCl pH 7.4, and clarified by centrifugation at 3220 g for 30 min at 4 C

Investig Ophthalmol Vis Sci 46:349357

Sigma-Aldrich) was added to neat DMSO and added to molten NGM at 55C to a final concentration of 5, 10, 15 or 20 mM DEM and 0.5 % v/v DMSO

The preparation method comprises the following steps of: (1) adopting p-nitrobenzoic acid as a starting material, adopting oxalyl chloride as an acylating chlorination reagent, adopting tetrahydrofuran and DMF (Dimethyl Formamide) as a mixed solvent, and carrying out acylating chlorination reaction to prepare paranitrobenzoyl chloride