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Enabling scientists to use human cells in their research, running additional experiments without rationing cells or limiting experimental scale Flow cytometry analysis demonstrates gene repression of CD81 upon lentiviral guide RNA delivery Repression levels depend on the chosen target and gRNA design No silencing of the transcriptional repression domain CRISPRi-Ready ioGlutamatergic Neurons form structural neuronal networks by day 11 CRISPRi-Ready ioGlutamatergic Neurons express neuron-specific markers CRISPRi-Ready ioGlutamatergic Neurons demonstrate gene expression of neuronal-specific and glutamatergic-specific markers following deterministic cell programming The dedicated User Manual for the CRISPRi-Ready ioGlutamatergic Neurons must be used for culture and gRNA delivery, and not the user manual for the wild-type ioGlutamatergic Neurons (io1001)

degrading effective amount refers to the amount of enzyme which is required to process at least 50% of the substrate, as compared to substrate not contacted with the enzyme

4-OXO-DHA was the most potent

T cells, particularly Th2 cells, provide co-stimulatory signals by binding CD40L on their surface to CD40 on B cells, promoting B cell differentiation into plasma cells and antibody production (147)

doi: 10.1016/S1074-7613(01)00218-7 57 EvelynAKJLeisaMConstanceWAlisonPKerriGPeterEet al