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Genome-wide identification of glutathione S-transferase and expression analysis in response to anthocyanin transport in the flesh of the new teinturier grape germplasm zhongshan-HongYu

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169 These consisted in low mitotic activity in glands and stroma, unusually thick-walled muscular arterioles, and prominent aggregations of thin-walled vessels in the stroma

Ran activation assay Levels of RanGTP were assessed by immunoprecipitation using RanBP1(RBD)-GST fusion proteins bound to glutathione-sepharose beads, which selectively isolate the active form of Ran (RanGTP) in the lysis Buffer: 25 mM HEPES, Ph 7.5, 150 mM NaCl, 1% NP-40, 10 mM MgCl2, 1 mM EDTA, 2% Glycerol, 10 mM NaF, protease inhibitor tablet (Roche), followed by western blot analysis with anti-Ran antibody