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The reaction was stopped by adding ice-cold methanol (1:2 v/v), the mixture was centrifuged 10,000 g for 10 min to remove proteins, the supernatant was mixed with 0.1% acetic acid and loaded onto a solid-phase lipid extraction cartridge (Oasis PRIME HLB cartridge (60 mg, 3 cc))

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The extraordinary fu organs are a collective term for the brain, marrow, bone, vessels, gallbladder, and the n-zi-bao (, usually indicating the uterus)

Zakka K, Chidambaram S, Mansour S, Mahawar K, Salminen P, Almino R, et al

Fumagalli et al., As with FcRs, SLP-76, PLC2, and Vav are also required for ROS production downstream of integrin stimulation (Myung et al., 2001