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Genome-wide meta-analysis finds the ACSL5-ZDHHC6 locus is associated with ALS and links weight loss to the disease genetics

NOV11 Sequence Analysis SEQ ID NO: 47 1024 bp NOV l la, GACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTCAC CG I 10725- TACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCA 01 DNA GTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAG ATGCTGTGGCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCC Sequence ACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGT AAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACC ATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGA TGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACT GATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAG ACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAA GTTTCGCAGACCTGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACAC ATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGA ACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGA TGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAAAGTCAGCCGTGAATTCCAC AGCCATGAATTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAG AAGATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGT CAATTAAAAGGAGAAAAAAATACAATTTCTCACTTTGCATTTAGTCAAAAGAAAAAAT GCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCT ORF Start: ATG at 63 ORF Stop: TAA at 933 SEQ ID NO: 48 290 aa MW at 32606.4 Da NOV l la, MRIAVICFCLLGITCAIPVKQADSGSSEEKQ YNKYPDAVAT NPDPSQKQNL APQ CG I 10725- NAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDD 01 Protein SHQSDESHHSDESDELVTDFPTDLPATEVFTPWPTVDTYDGRGDSWYGLRSKSKKF RRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDD Sequence QSAETHSHKQSKVSREFHSHEFHSHEDMLWDPKSKEEDKHLKFRISHELDSASSEVN Further analysis of the NOVl la protein yielded the following properties shown in Table 1 IB