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The preparation of GSH Since 1938, the first patent for the use of yeast GSH has been published, and a large number of patent applications have since been published.In general, the preparation methods of GSH are solvent extraction, enzymatic, fermentation and chemical synthesis of four.At present, mainly from the cultivation of high content GSH yeast extract, domestic manufacturers have Shanghai yeast plant and a number of institutions, research units are being developed, foreign manufacturers are BDH, Fluka, J.T.Baker, E.Merck, Haen Riedel-de, Siqma and Japan and the light of the public.The extraction method and enzyme method are mostly wheat germ as raw material, by adding appropriate solvent or combined with amylase, protease treatment, and then by centrifugal, separation and purification

To initiate biofilm growth, planktonic bacteria were re-suspended in TSB and 250 L of bacterial cell suspension (OD 600 = 0.5 0.05) was added into the wells of 96-well plates (Corning Corp