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Depletion of intracellular glutathione (GSH) markedly promotes mitochondrial ROS production and triggers mitochondrial membrane depolarization (Lohan et al., 2018)

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DMG levels positively correlated with methoxyestrogens and methoxyestrogen: hydroxyestrogen ratios in COC users, while S-adenosylmethionine (SAM) negatively correlated with 2-MeOE2

The ROC curve for these characteristic metabolites exhibited substantial sensitivity (AUC = 0.929) (Figure 8C)

PCR positive plants were later transferred to insect-proof cages for insect bioassays