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After incubation, 1 ml of 4 mM methimazole (MMI) was added and cells were washed with ice-cold PBS

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This process promotes the sequestration of calcium ions in the sarcoplasmic reticulum while reducing their concentration in the cytosol, which impacts phosphorylation and consequently results in smooth muscle relaxation (Fig

doi: 10.1002/med.2016.36.issue-5 36 MatthaiouC