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UV-Vis absorption spectra was acquired by TU-1810 spectrometer (Purkinje, China)

For a quantitative insight, this finding was further confirmed by flow cytometry (flow cytometry plots: Fig

The use of transgenic fluorescent lines, such as Tg (fabp10a: eGFP), significantly enhances sensitivity, allowing for real-time, non-invasive monitoring of hepatic integrity by quantifying fluorescence attenuation, morphological contraction, or changes in fluorescence intensity ( il-1 , tnf- , il-6 ) help elucidate the cellular pathways driving damage (Marginean, 2025