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Description

Int J Obes Suppl 10(1):5061

Feng Q, Liu D, Lu Y, Liu Z

Alterations in these indices were further supported by ultrastructural studies carried out in the brain as indicated by myelin disintegration , cell organelle alterations and neuronal loss by mercury poisoning

Apoptosis To detect apoptotic nuclei, TUNEL staining was performed using an ApopTag Red In situ Apoptosis Detection Kit (Millipore, S7165, Darmstadt, Germany) according to the manufacturers instructions

The non-specific binding sites were blocked with 5% nonfat milk in TBST (10 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 8) for 1 h at room temperature