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GST fusion proteins on beads were incubated with recombinant proteins at 25 C for 2 hours or 4 C overnight in reaction buffer (25 mM Tris-HCl pH 7.4, 50 mM KCl, 2.5 mM Mg(OAc) 2 , and 0.1 % Triton X-100)

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The endpoints included the oxidative stress marker thiobarbituric acid-reactive substances (TBARS), plasma osteopontin (OPN), plasma paraoxonase (PON1) activity, H&E histology, gadolinium-enhanced magnetic resonance imaging (MRI) of leg muscle and motor functional measurements

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