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Meanwhile, we used high-performance liquid chromatography (HPLC) to quantify the generated Fluo under different treatments, which showed the more Fluo production in TYR-catalyzed group compared to copper sulfate/SA group (Supplementary Fig

Interestingly, a number of intracellular proteins that can be released and act as endogenous inflammatory signalling molecules (also known as damage-associated molecular patterns) can undergo glutathionylation, including high mobility group box 1 protein (HMGB-1) 14 , proteins of the S100 family 15 , galectin-1 16 , peroxiredoxin-2 (Prdx2) 17 and heat-shock protein 70 (HSP70) 18

If you add test material which possesses reducing properties, please prepare a few wells that only contain the medium without cells and only test material for background control

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