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Immunoblotting For adherent cell lines, cells were plated in six-well or 12-well plates at approximately 1 10 6 cells per well and allowed to adhere overnight

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Despite the limitations associated with identifying bona fide protein-protein interactions by coupling co-immunoprecipitation or proximity labeling with LC/MS-MS, we were able to confidently generate a shorter, more manageable list of 19 potential interacting kinases, predominantly intestinal, for further study

elegans ) in response to changes in the environment