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Understanding that purging is normal (and often a sign the treatment is working) helps users push through this uncomfortable phase rather than abandoning treatment prematurely

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On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature

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Serious cardiac events, including some that have been fatal, have occurred following use of the parenteral form of dihydroergotamine mesylate, but are extremely rare