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Amplification fragments were assembled using Gibson assembly with Pst I-HF (NEB, R3140S) and Kpn I-HF (NEB, R3142S)-digested pUC18R6K-mini-Tn7T-Gm and transformed into One Shot PIR1 chemically competent E

B12 also plays an important part in maintaining nerve health

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To further elucidate the regulatory mechanisms of the key genes, molecular docking and molecular dynamics simulations were performed on gut metabolites influencing these genes, assessing their binding affinity and stability