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Vitamin E & -carotene (fat soluble), for example, protect against free radicals targeting the inner layer of the cell membrane, while vitamin C (water soluble) protects only the outer layer

doi: 10.1016/j.etap.2024.104391 [DOI] [PubMed] [Google Scholar] 24

DAB was used as the chromogen

In addition, it inhibits lipoxygenase activity (Georgieva et al., 2013) and was shown to bind iron ions and to reduce Fe 3+ to Fe 2+ (Binbuga et al., 2005)