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Measurement of the large intestine length, and hematoxylin and eosin (HE) staining, and immunohistochemistry Under deep anesthesia using a combination anesthetic (0.3 mg/kg medetomidine, 4.0 mg/kg midazolam, and 5.0 mg/kg butorphanol) 56 , DSS-induced colitis mice were perfused with 4% paraformaldehyde (PFA) in 0.01 M phosphate buffer (PB, pH 7.4)

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The second part of the study involved administering oral folinic acid 800mcg and 1000mg betaine twice daily