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OsDHAR was purified by Ni-NTA affinity chromatography [5 mL HisTrap FF column (GE Healthcare, Little Chalfont, UK) on an AKTA FPLC system] and eluted with a step gradient of 250 mM imidazole

This increase in pH, shuts down the enzymes that drives melanin production

Ferroptosis vulnerability of immune cells Apart from cancer cells, TME also comprises immune cells, including T cells, macrophages, myeloid-derived suppressor cells (MDSCs), etc