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They are called Blue Zones because when Buettner and his colleagues were searching for these areas, they drew blue circles around them on a map

The full-text of the study is available free on his web site

Abbreviations AP alkaline phosphatase CDO cysteine dioxygenase FBS fetal bovine serum GCL glutamate-cysteine ligase LDH lactate dehydrogenase MTT 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide PLP pyridoxal phosphate pNPP p-nitrophenyl phosphate RRID Research resource identifier SVZ subventricular zone TNAP Tissue-nonspecific alkaline phosphatase TSP sodium 2,2,3,3-tetradeutero-3-trimethylsilylpropionate VZ ventricular zone References 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46

By bypassing the digestive system, IV therapy delivers essential nutrients and vitamins directly into the bloodstream without being processed and diluted by the stomach and intestines

As used herein, the terms "gene" and "recombinant gene" refer to a nucleic acid molecule comprising an open reading frame encoding a polypeptide corresponding to a marker encompassed by the present invention