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Description

For specific staining of the dead cells after expression of WD-associated G53S or M128V PrP variand, control, and treated cells were washed in warm media and incubated for 1 h at 37 C in the incubator with BOBO3 (Life Technologies) diluted 1:1000 in the media

To distinguish these two likely causes, we performed: 1) a sliding-window (20 Kb size) individual clustering analyses of absolute divergence along the genome employing a phylogenetic tree based on the concatenated sequences