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Although direct clinical evidence for their pulmonary protective effects is still accumulating, meta-analyses of large cardiovascular outcome trials indicate that SGLT-2 inhibitors significantly reduce the risk of acute exacerbations in diabetic patients with comorbid COPD (Foer et al., 2023), suggesting that they provide a pleiotropic organ protection beyond hypoglycemic effects

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FLAG-tagged BRD4 proteins were incubated with 500 ng of refolded RNA while rotating at 4 C for 1 h in RNA binding buffer (20 mM Tris-HCl at pH 7.4, 100 mM KCl, 0.2 mM EDTA, 0.05% NP40, 0.4 U RNase inhibitor, PICs)