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Description

Mitochondria lysates were prepared from mitochondria derived from heart samples by centrifugation

2.8 Immunohistochemical analysis Paraffin sections from different groups of liver tissues from rats were stained by immunohistochemistry (IHC) according to the manufacturing protocol using Anti-caspase 3 antibody (ab4051), at dilution 1:1,000, Anti-Bax antibody (ab32503), at dilution 1:250 and Anti-Bcl-2 antibody (ab182858), at dilution 1:500, (abcam, Cambridge, United Kingdom)