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Thus, as the SLC1A5 variant is the only currently known glutamine transporter in the mitochondrial inner membrane 16 , targeting the SLC1A5 variant could be an effective strategy for selectively inhibiting glutamine metabolism in cancer cells (Fig

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For flow cytometry experiments, cells were prepared as indicated in the flow cytometry section and incubated for 30 min at 37 C with Calcein-AM (10 M) or Rhonox (5 M) before being washed twice with PBS and suspended in PBS containing 0.5% serum prior to being analyzed