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This way method, the dye ligand is linked to by a covalent bond

doi: 10.1038/nrm2688 99 VoinnetO

2.9 Proteomic data visualization and trend analysis Protein abundance ratios between groups were calculated from label-free quantification values and log 2 -transformed prior to visualization

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In methionine choline-deficient diet-induced non-alcoholic steatohepatitis mice, esculin (20-40 mg/kg) also inhibits the NF-B signaling pathway, downregulates the expression of TNF- and IL-6, and increases the expression level of the silent information regulator 1(99)