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The formation of TNB was read against the blank in a UV-Visible double beam spectrophotometer (Shimadzu Corporation, Tokyo, Japan) at 412 nm

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While the average lifespan didnt change, the longest-living mice lived significantly longer

A temperature gradient was set in the thermocycler for LacAnc100 and LaccID ranging from 30 to 70 C as follows (in C): 30.0, 30.9, 32.7, 35.8, 39.4, 41.8, 43.7, 45.0, 46.8, 49.8, 54.4, 59.9, 64.8, 68.0 and 70.0

5-Amino-1MQ binds the NNMT active site at low micromolar Ki, producing selective inhibition relative to other SAM-dependent methyltransferases [1]