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An elevation in MDA levels indicates oxidative stress caused by various substance, including xenobiotics 74

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2.7 Size Exclution Chromatography The protein sample was concentrated to 1 mL and loaded onto a Superdex 200 10/300 GL column (GE Healthcare) with gel filtration buffer (20 mM TrisHCl (pH 8.0), and 200 mM NaCl) using an KTA purifier (GE Healthcare)

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Glutamate group was given glutamate (4 mg/kg or 8 mg/kg) gavage continuously for 3 days, in the same way, the rats were positioned in a cylindrical rat retainer at 4C for 3.5 h then all the rats were sacrificed under anesthesia with 10% chloral hydrate (0.3 ml/100 g), and gastric juice was collected