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The resulting cell lysate was filtered through a 0.2-m filter and loaded onto a 5-mL HisTrap HP column (GE Healthcare/Cytiva) equilibrated in wash buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 25 mM imidazole)

10.1016/j.exer.2015.04.016 75 Medvidovic-GrubisicM.StambolijaV.KolencD.KatancicJ.MurselovicT.Plestina-BorjanI.et al (2017)

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Thus, HGF levels could be employed as a biomarker for disease status/progression, and HGF/c-Met signaling pathway modulators could effectively regulate IR and treat diabetes