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doi: 10.1111/tpj.12490 Summary Keywords tetrapyrrole biosynthesis, chlorophyll metabolism, redox control, thiol-disulfide switch, thioredoxin Citation Sinha N, Hussein R, Paul J, Nazare M and Grimm B (2025) Redox regulation of glutamate-1-semialdehyde aminotransferase modulates the synthesis of 5-aminolevulinic acid in Arabidopsis

Build protocols around compounds with stable supply chains when possible

To generate the initial anti-MBP IgG construct, total RNA was isolated from 4 10 7 hybridoma cells using a Qiagen RNeasy kit with the following modifications: 4 10 7 cells were resuspended in 600 l RLT buffer, homogenized with hand-held homogenizer for 2 min followed by the addition of 1,800 l RLT buffer (2,400 total, 600 l per 1 10 7 cells), mixed, separated into 4 600-l aliquots and homogenized for another 30 s

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We also provide a bifurcation analysis analysis with respect to an important parameter here