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Samples were then sonicated 5 s for 5 pulses at 70% power four times (the sonicator is a HD 2070 from Bandelin Sonoplus equipped with a MS73 probe) and then centrifuged at 12,000 g for 10 min at 4C

The signal amplification mechanism was demonstrated by assessing the influences of various factors such as MB concentration, BSA concentration, solution pH and incubation temperature 14

While studies (50,73,74,151,82) have reported ChaC1/ChaC2 as tumor biomarkers, the development of ChaC1/ChaC2 tracer fluorescent probes remains unexplored