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To quantify the expression of S-100, GDNF, and BDNF in transplanted human GMSCs, the area of colocalized immunolabeling signals (in yellow-orange pixels) of these proteins (in green color) and human nuclei (in red color) in the merged files was measured using ImageJ program and presented as the percentage of colocalization = the area of yellow-orange pixels/total area of green pixels [42]

Gagnon, D., Jay, O

Axons that touch the dashed lines and are totally inside the unbiased counting frame were counted

Then, we continue for one more hour to the starting point

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